Unique Red Blood Cell Morphology Detected in a Patient with Myelodysplastic Syndrome by Three-dimensional Refractive Index Tomography

The three-dimensional (3-D) shape of erythrocytes is strongly associated with various diseases. However, conventional optical imaging approaches with Wright's staining only provide information on two-dimensional morphology. Here, we employed optical diffraction tomography (ODT), a label-free 3-D quantitative phase imaging technique, and observed uniquely shaped red blood cells (RBCs) in the peripheral blood of a patient diagnosed with myelodysplastic syndrome. Peripheral blood samples were collected when the patient visited our hospital for his two out-patient follow-ups in May 2018. The 3-D tomograms of randomly chosen RBCs were reconstructed using a commercial ODT setup. From the reconstructed 3-D RBCs, 37.5% and 32.8% of RBCs demonstrated cup-like shapes at the first and the second out-patient follow-up, respectively. Even though this is a single case report, the finding is novel and can be a potential dyserythropoietic feature found in peripheral blood.

Study on measuring method for surface micromorphology of human incisor / 医用生物力学

Objective To develop a microscopic 3D morphology testing system used for measuring the natural texture of human incisor, and provide practical data for the individualized technique used in dental restoration. Methods Based on microscope and digital image correlation (DIC), a microscopic 3D morphology testing system with its software was developed. The precision of this system was then evaluated, and the characterization of the 3D surface morphology for human incisor was measured by the system. Results Within the field of view (FOV) of 17 mm×12 mm, the accuracy of in plane displacement was estimated as 0.6 μm, while that of out of plane displacement was 5.4 μm. The dimension of horizontal grooves on the labial surface of incisor was determined, where the depth and length for the long groove were 83 μm and 5.28 mm, respectively; the depth and length for the short groove were 40 μm and 4.24 mm, respectively, and the space between the two grooves was 2.85 mm. Conclusions The microscopic 3D morphology testing system is an effective instrument for measuring the surface morphology of human incisors accurately.